Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Improved Efficacy of a Dendritic Cell-Based Vaccine against a Murine Model of Colon Cancer: The Helper Protein Effect

doi: 10.4143/crt.2013.241

Figure Lengend Snippet: Verification of gp70 expression in CT26 cells and tumors. (A-D) Immunofluorescence staining. CT26 cells showed positive staining with rabbit-anti-mouse AH1 antibody (green cytoplasm). (A) The nuclei were stained using 7-AAD (red). (B) Murine 4T1 cell line served as the negative cell control. (C) CT26 cells were stained with peptide-adsorbed Ab. (D) CT26 cells were stained with rabbit IgG as the negative reagent control (A-D, ×40). (E) Western blot analysis of gp70 expression. 1, CT26 cell lysate; 2 and 5, CT26-derived tumor lysate; 3, murine 4T1 cell lysate (as the negative cell control); 4, normal murine muscle tissue lysate (as the negative tissue control); 6, CT26 cell lysate treated with rabbit IgG (as the negative reagent control); 7, CT26 cell-derived tumor lysate treated with rabbit IgG (as the negative reagent control). The 90 kDa band corresponds to gp90, the precursor of gp70.

Article Snippet: CT26 mouse colon carcinoma cells were purchased from the Pasteur Institute, Iran, and used for this study.

Techniques: Expressing, Immunofluorescence, Staining, Control, Western Blot, Derivative Assay

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Improved Efficacy of a Dendritic Cell-Based Vaccine against a Murine Model of Colon Cancer: The Helper Protein Effect

doi: 10.4143/crt.2013.241

Figure Lengend Snippet: Effect of various DC-based vaccination protocols on tumor size. Mice were inoculated subcutaneously with CT26 tumor cells (day 0). On days 3 and 10, mice were injected with DCs loaded with different Ag mixtures, shown in the figure. The tumor volume in all vaccination groups was measured from day 0 at 3-day intervals for 42 days. DC, dendritic cell; Ag, antigen; OVA, ovalbumin. a) As of day 18, the average tumor volume in tumor-bearing mice vaccinated with either DC-Pep or DC-Pep-OVA was significantly smaller than in those treated with DCP15-OVA, with naïve DC (DC-alone), or not treated at all (tumor), b) From day 36 and beyond, a significant difference in terms of tumor size was observed between the DC-Pep and DC-Pep-OVA groups. Data are shown as the average tumor volume of five mice per group on each day.

Article Snippet: CT26 mouse colon carcinoma cells were purchased from the Pasteur Institute, Iran, and used for this study.

Techniques: Injection

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Improved Efficacy of a Dendritic Cell-Based Vaccine against a Murine Model of Colon Cancer: The Helper Protein Effect

doi: 10.4143/crt.2013.241

Figure Lengend Snippet: The therapeutic impact of DC-based vaccination on the survival rate of tumor-bearing mice. Groups of 10 mice were inoculated subcutaneously with CT26 tumor cells (day 0). On days 3 and 10, mice were injected with DCs loaded with different Ag mixtures, shown in the figure. The survival rate of all groups was determined weekly for 10 weeks and expressed as the percentage of live mice per treatment group. Significantly higher survival rate was observed in DC-Pep-OVA-treated mice compared to those vaccinated with DC-Pep. DC, dendritic cell; Ag, antigen; OVA, ovalbumin.

Article Snippet: CT26 mouse colon carcinoma cells were purchased from the Pasteur Institute, Iran, and used for this study.

Techniques: Injection

Journal: Frontiers in Pharmacology

Article Title: Natural Polysaccharides and Their Derivates: A Promising Natural Adjuvant for Tumor Immunotherapy

doi: 10.3389/fphar.2021.621813

Figure Lengend Snippet: The immunoregulatory activity of natural polysaccharides and their derivates on immune cells.

Article Snippet: , C57BL/6 (6 weeks old), BALB/c, OT-I and OT-II TCR transgenic mice and C57BL/6-Ly5.1 (CD45.1) congenic mice; TLR2, TLR4 and SR-A-KO mice; the murine melanoma cell line B16F10 (ATCC, CRL-6475) expressing OVA (B16-OVA) and murine carcinoma cell line CT26 (ATCC, CRL-2639) , Increase levels of co-stimulatory molecule expression and pro-inflammatory cytokine production in spleen DCs dependent on TLR4; enhance ovalbumin (OVA) antigen (Ag)-specific immune activation in tumor-bearing mice , .

Techniques: Activity Assay, Functional Assay, Expressing, Activation Assay, Membrane, Gene Expression, In Vivo, Protein-Protein interactions, Concentration Assay, Transgenic Assay, In Vitro, Phospho-proteomics, Isolation, Cell Culture, Modification, Control, Algae

Journal: Frontiers in Microbiology

Article Title: Gut Microbiome Influences the Efficacy of PD-1 Antibody Immunotherapy on MSS-Type Colorectal Cancer via Metabolic Pathway

doi: 10.3389/fmicb.2020.00814

Figure Lengend Snippet: Effect of PD-1 antibody immunotherapy on the diversity of CT26 tumor-bearing mice with different gut microbiome. (A) Schematic diagram of mouse model experiment process and sample collection and analysis. (B) Tumor size of mice at the end of immunotherapy. (C) Tumor size of mice at the end of immunotherapy in the presence of different antibiotic regimen in each group.

Article Snippet: The MSS-type mouse colon carcinoma cell lines colon 26 (CT26) (purchased from Procell Life Science & Technology Co., Ltd. Cat:CL-0071) were cultured in RPMI1640 medium (GIBCO) supplemented with 10% heat-inactivated fetal calf serum at 37°C in a 5% CO 2 incubator.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Gut Microbiome Influences the Efficacy of PD-1 Antibody Immunotherapy on MSS-Type Colorectal Cancer via Metabolic Pathway

doi: 10.3389/fmicb.2020.00814

Figure Lengend Snippet: Gut microbiome diversity is associated with response to PD-1 antibody immunotherapy in CT26 tumor-bearing mice. (A) Venn diagram of the total number of species shared between the three groups. (B) Hierarchical clustering tree on OUT level. (C) The community map of dominant species in three group at the level of species, the columns with different colors represent different species, and the length of the columns represents the proportion of species. (D) Bar plot of compositional differences at species level in the gut microbiome of three groups of mice by one-way ANOVA. (E) Circos of sample and species: a visual circle diagram describing the correspondence between samples and species.

Article Snippet: The MSS-type mouse colon carcinoma cell lines colon 26 (CT26) (purchased from Procell Life Science & Technology Co., Ltd. Cat:CL-0071) were cultured in RPMI1640 medium (GIBCO) supplemented with 10% heat-inactivated fetal calf serum at 37°C in a 5% CO 2 incubator.

Techniques: